Microwave-Assisted Green Synthesis of Punica Granatum Peel–Mediated Silver Nanoparticles with Potent Antibiofilm Activity

Author(s)	Ruqya Banu, D. Narmada
Country	India
Abstract	Silver nanoparticles (AgNPs) were successfully synthesized using Punica granatum peel extract via a microwave-assisted biogenic method. This green synthesis approach is rapid, cost-effective, and environmentally friendly, avoiding toxic chemicals and harsh reaction conditions. Formation of AgNPs was confirmed by a visible color change and a characteristic surface plasmon resonance (SPR) peak at 410–430 nm in UV–Visible spectra. Synthesis parameters, including microwave irradiation time and reactant concentrations, were optimized to enhance nanoparticle yield and stability. Characterization using UV–Vis, FTIR, DLS, XRD, TEM exposed well-dispersed, spherical AgNPs with an average size of 8 ± 2nm, a low polydispersity index (0.224), and a zeta potential of −30.5mV, indicating good colloidal stability. Phytochemicals in peel extract acted as reducing as well as stabilizing agents. The synthesized AgNPs exhibited significant antibiofilm activity, highlighting their potential for biomedical and antimicrobial applications.
Published In	Conference / Special Issue (Volume 17 \| Issue 1) - One Day National Seminar on “Advances in Life Sciences for Diversity, Applications, and Human Welfare” (ALSDAHW-2025) (March 2026)
Published On	2026-03-16
DOI	https://doi.org/10.71097/IJSAT.ALSDAHW-2025.121

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