International Journal on Science and Technology

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Volume 17 Issue 1
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Phytochemical identification of compounds using HR-LCMS analysis and antioxidant as well as antibacterial activity of aqueous extracts of Seaweeds.

Author(s) Dr. SRUTHY E P M, Dr. Chitra G
Country India
Abstract The diverse array of phytoconstituents in marine macro algae highlights their potential as functional foods and sources of natural bioactive compounds for health applications. The aim of this study was to find different bioactive compounds and evaluate their antioxidant qualities and antimicrobial effects in Gracilaria corticata. J. Ag., Padina tetrastromatica Hauck and Caulerpa peltata. Lamour. The process included gathering the seaweed, washing it with flowing water, drying it in the shade and then extracting it with water. The phytochemical analyses conducted on the three varieties of seaweeds showed the existence of phenols, flavonoids, alkaloids, saponins, tannins and glycosides, through both qualitative and quantitative methods. Nonetheless, terpenoids were not present in Gracilaria. HRLC-MS was utilized to examine the phytochemical compounds found in the seaweed. The disc diffusion technique was used to evaluate the antimicrobial efficacy of seaweed extracts against Staphylococcus aureus and Klebsiella pneumoniae. HR-LCMS analysis revealed diverse compounds in both the positive and negative chromatogram. The water extract from Gracilaria corticata showed higher antioxidant activity than the other extracts evaluated. The largest areas of inhibition were seen in the presence of the gram-positive bacteria Staphylococcus aureus (15.33±1.15) and gram-negative bacteria Klebsiella pneumoniae (20.67±1.15).
Keywords Key words: Seaweeds; phytoconstituents; Antioxidant; HRLC-MS; Anti-bacterial
Field Biology > Agriculture / Botany
Published In Volume 17, Issue 1, January-March 2026
Published On 2026-02-28
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