In vitro Antimicrobial Activity of Aqueous Root Extract of Hygrophila auriculata against Staphylococcus aureus and Escherichia coli

Sourav Saha; Laxmipriya Baskey; Manmeet kaur; Anuta Sarkar

doi:10.71097/IJSAT.v17.i2.11255

In vitro Antimicrobial Activity of Aqueous Root Extract of Hygrophila auriculata against Staphylococcus aureus and Escherichia coli

Author(s)	Mr. Sourav Saha, Ms. Laxmipriya Baskey, Ms. Manmeet kaur, Ms. Anuta Sarkar
Country	India
Abstract	The growing emergence of antimicrobial resistance has created a need for the discovery of effective and safer alternatives from natural sources. The present study was undertaken to investigate the pharmacognostic characteristics, phytochemical constituents, and in vitro antibacterial activity of the root of Hygrophila auriculata (Kokilaksha), a medicinal plant widely used in traditional Indian medicine. The roots were collected, authenticated, dried, powdered, and subjected to physicochemical evaluation and aqueous extraction. Preliminary phytochemical screening was carried out to identify major classes of bioactive compounds. The antibacterial activity of the aqueous root extract was evaluated against Staphylococcus aureus and Escherichia coli using the agar well diffusion method, while the Minimum Inhibitory Concentration (MIC) was determined by broth dilution technique. The extract exhibited concentration-dependent antibacterial activity against both test organisms. The maximum zone of inhibition was observed at 100 mg/mL, while MIC values were found to be 75 mg/mL for S. aureus and 100 mg/mL for E. coli. The higher sensitivity of S. aureus indicated better activity against Gram-positive bacteria. The antimicrobial effect may be attributed to the presence of phytoconstituents such as flavonoids, tannins, saponins, alkaloids, and phenolic compounds, which are known to interfere with microbial growth through multiple mechanisms. Although the activity was lower than the standard antibiotic, the findings suggest that Hygrophila auriculata root possesses promising antibacterial potential and may serve as a valuable source for the development of plant-based antimicrobial agents. Further studies involving isolation and characterization of active constituents are recommended.
Keywords	Hygrophila auriculata, Kokilaksha, Antibacterial Activity, Medicinal Plant, Phytochemical Screening, Aqueous Root Extract, Agar Well Diffusion, Minimum Inhibitory Concentration (MIC), Staphylococcus aureus, Escherichia coli, Antimicrobial Agents, Pharmacognosy
Field	Medical / Pharmacy
Published In	Volume 17, Issue 2, April-June 2026
Published On	2026-06-09
DOI	https://doi.org/10.71097/IJSAT.v17.i2.11255

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In vitro Antimicrobial Activity of Aqueous Root Extract of Hygrophila auriculata against Staphylococcus aureus and Escherichia coli

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