In Vitro Anti-Bacterial activity of Hibiscus Sabdariffa L Stem in Acetone Extract

Author(s)	Prof. Dr. Nagaraja Ganapati Tarikere, Ms. Shreya Mohan Zambare, Ms. Sakshi Vijay Gondhali, Ms. Madhura Wali
Country	India
Abstract	The present investigation envisages anti-bacterial activity of Hibiscus sabdariffa L stem in invitro condition. The extraction was carried out by Soxhlet apparatus using acetone as solvent. The antibacterial activity was measured by well diffusion method as prescribed by Allice and Sivaprakasam (1966) using test bacteria such as Bacillus megaterium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella typhimurium. The zone of inhibition was recorded in cm. A maximum zone of inhibition was recorded (2.53cm) against Pseudomonas aeruginosa indicates stem extract possess a considerable number of bactericidal compounds as compared to control – acetone solvent. After Pseudomonas aeruginosa, Salmonella typhimurium shows a recognizable zone of inhibition as (2.5) cm followed by Escherichia coli (2.17 cm). Hence crude extract of acetone reflects a good bactericidal property; may be due to presence some phytochemicals.
Keywords	Hibiscus sabdariffa L, Bacillus megaterium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhimurium
Field	Biology > Agriculture / Botany
Published In	Volume 17, Issue 3, July-September 2026
Published On	2026-07-18

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