International Journal on Science and Technology
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Volume 17 Issue 3
July-September 2026
Indexing Partners
Development of Leaf Extract Spectral Database for Plant Species Classification Using Liquid Light Guide Spectroscopy
| Author(s) | Gaju S. Chavan, Dr. Sonali B. Kulkarni, Simran N. Maniyar |
|---|---|
| Country | India |
| Abstract | Plant species identification is crucial in agriculture, conservation of biodiversity, forestry, medicinal plant authentication and precision farming. The traditional identification using morphological features is often time consuming, requires expert knowledge and has limitations due to environmental changes and seasonal variations. In this study, developed a standard library of leave extracts spectra for automatic plant species identification using transmission spectroscopy. Fresh, healthy leaves were used to prepare leaf extracts which were developed spectral library using the ASD FieldSpec4 spectroradiometer with Liquid Light Guide (LLG) over the spectral range of 350–2500 nm in the visible (VIS), near infrared (NIR), and short-wave infrared (SWIR) spectral regions. Several biological samples were taken for each plant species and 10 spectra were recorded for each sample after calibration with dark and blank samples to increase the reliability of the measurements. The recorded spectra were averaged and prepared in a hierarchical spectra database based on species, biological material and repeated spectrum acquisition. Proposed transmission spectroscopy is a different method to conventional reflectance spectroscopy, which is able to measure biochemical signatures related to pigments, water, carbohydrates, proteins and other metabolites in leaf extracts. This spectral database will be use as a credible basis for the spectral pre-processing, machine learning and plant species classification based on artificial intelligence. This effort provides a standardized dataset that facilitates the development of strong, repeatable and non-destructive plant identification systems for future use in the agricultural and environmental domains. |
| Keywords | : Liquid Light Guide, Spectral Transmission, biodiversity, Plant Species, Spectroscopy. |
| Field | Computer Applications |
| Published In | Volume 17, Issue 3, July-September 2026 |
| Published On | 2026-08-02 |
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Crossref DOI prefix of IJSAT is 10.71097/IJSAT
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