International Journal on Science and Technology

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Phytochemical Profile and Antioxidant Capacity of Aqueous Hibiscus tiliaceus L. Flower-Petal Extract: Relevance to Green Silver Nanoparticle Synthesis

Author(s) Mr. Panagupalli Prasad Kumar, Prof. Sudhakar Poda
Country India
Abstract The present study characterized the phytochemical composition and antioxidant potential of an aqueous flower-petal extract of Hibiscus tiliaceus L. as a biochemical basis for its subsequent use in plant-mediated silver nanoparticle synthesis. Fresh petals were shade-dried, powdered and extracted with water (1:20, w/v) at 60 °C for 30 min. Qualitative phytochemical screening, total phenolic content (TPC), total flavonoid content (TFC), and DPPH and ABTS radical-scavenging assays were performed. Phenolics, flavonoids, saponins, carbohydrates, reducing sugars, proteins, free amino acids, lipids/fixed oils, alkaloids, terpenoids and anthraquinones were detected, whereas tannins, steroids, cardiac glycosides, coumarins and ester-containing constituents were not detected under the applied screening conditions. TPC increased from 8.29 ± 0.91 to 19.14 ± 1.10 mg GAE/g extract as the petal quantity increased from 1 to 3 g, while TFC increased from 6.31 ± 0.48 to 14.71 ± 0.88 mg QE/g extract. The petal extract displayed marked concentration-dependent antioxidant activity. DPPH scavenging increased from 3.29 ± 0.04% at 10 µg/mL to 89.88 ± 3.04% at 400 µg/mL, whereas ABTS scavenging increased from 2.77 ± 0.06% to 88.10 ± 2.66% over the same range. The corresponding IC50 values were 166.40 and 177.35 µg/mL, respectively. Ascorbic acid showed lower IC50 values (19.36 and 21.08 µg/mL), as expected for a purified reference antioxidant. The combined phytochemical and antioxidant findings demonstrate that H. tiliaceus petals contain a redox-active aqueous phytochemical matrix with potential to support metal-ion reduction and surface stabilization in green nanoparticle synthesis.
Keywords Hibiscus tiliaceus; flower petals; phenolics; flavonoids; DPPH; ABTS; antioxidant activity; green synthesis.
Field Biology
Published In Volume 17, Issue 3, July-September 2026
Published On 2026-08-14
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Crossref DOI prefix of IJSAT is 10.71097/IJSAT

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