International Journal on Science and Technology

E-ISSN: 2229-7677   •   Impact Factor: 9.88

A Widely Indexed Open Access Peer Reviewed Multidisciplinary Bi-monthly Scholarly International Journal

Call for Paper Volume 16 Issue 2 April-June 2025 Submit your research before last 3 days of June to publish your research paper in the issue of April-June.
Submit Research Paper Join as a Reviewer

Plagiarism is checked by the leading plagiarism checker TurnItIn

Call for Paper

Volume 16 Issue 2
April-June 2025

Submit your research paper

Indexing Partners
Academia Advanced Sciences Index Bielefeld Academic Search Engine CiteSeer DRJI Google Scholar Independent Search Engine & Directory Network ISI (International Scientific Indexing) Issuu Mendeley Research Networks
RefSeek ResearcherId - Thomson Reuters ResearchGate Scirus Scribd Semantic Scholar UTeM - Universiti Teknikal Malaysia Melaka Wiki for Call for Papers WorldCat Zenodo

Development and validation of RP-HPLC method for Azelaic acid in prepared cubosomal dispersion using Design of Expert approach

Author(s) Kiran chenni, Anjana Adhyapak
Country India
Abstract Azelaic acid (AA), a naturally occurring dicarboxylic acid, is used extensively to treat acne and rosacea because of its antibacterial and anti-inflammatory qualities. The objective of this study was to create and validate a straightforward, reliable, and accurate RP-HPLC method for the measurement of AA in bulk and a novel cubosome formulation. Key chromatographic parameters, including the mobile phase composition (50:50 v/v of 0.5% formic acid: acetonitrile), flow rate (1 mL/min), and detection wavelength (210 nm), were chosen to produce the best retention time and resolution. The procedure was optimised using a Box-Behnken Design. The technique showed good accuracy (97–99% recovery), precision (%RSD < 2%), linearity (2–10 µg/mL, R2 = 0.995), and sensitivity (LOD: 1.521 µg/mL; LOQ: 3.342 µg/mL). Studies of forced deterioration under varied stress settings were used to establish stability-indicating capabilities. The verified technique was then used to measure AA in cubosomes, demonstrating favourable particle size (185 nm), zeta potential (-14.36 mV), and good drug entrapment effectiveness (92.4%). This HPLC approach provides a dependable tool for quality control and stability assessment of AA-based medicinal systems and is efficient for routine analysis of AA in pharmaceutical research and formulation development.
Keywords Azelaic acid, RP-HPLC, Cubosomes, QbD
Field Medical / Pharmacy
Published In Volume 16, Issue 2, April-June 2025
Published On 2025-05-21
DOI https://doi.org/10.71097/IJSAT.v16.i2.5314
Short DOI https://doi.org/g9mn9g
View / Download PDF File

Share this


CrossRef

CrossRef DOI is assigned to each research paper published in our journal.

IJSAT DOI prefix is
10.71097/IJSAT

Downloads
Research Paper Format Copyright Permission Form and Undertaking Form Cover Page Vol 15 Isu 4Cover Page Vol 15 Isu 3Cover Page Vol 15 Isu 2

All research papers published on this website are licensed under Creative Commons Attribution-ShareAlike 4.0 International License, and all rights belong to their respective authors/researchers.

CC-BY-SA Open Access

About IJSAT
Fees & Payment
Current Issue
Publication Archive 		Submit Research Paper
Track Submission Status
Publication Guidelines
Publication Ethics
Peer Review & Plagiarism 		Join as a Reviewer
Editors & Reviewers
Reviewer Referral Program
Get Reviewer Membership Certi. 		Website/Journal Policies
Usage Policy
Content Policies
Privacy Policy
Contact Us Message on WhatsApp +91-9687-182-185 editor@ijsat.org