In-vitro Antioxidant and Anti-Inflammatory Potential of herbal elixir containing Crocus Sativus and Nigella Sativa

Aisha Siddiqua; Nahida Siddiqui; Bhukya Kalyan; Bai Raveena; Tadikonda Rama Rao

doi:10.71097/IJSAT.v16.i2.6469

In-vitro Antioxidant and Anti-Inflammatory Potential of herbal elixir containing Crocus Sativus and Nigella Sativa

Author(s)	Aisha Siddiqua, Nahida Siddiqui, Bhukya Kalyan, Bai Raveena, Tadikonda Rama Rao
Country	India
Abstract	Background Oxidative stress and chronic inflammation are interlinked and associated with several major diseases, including cancer, cardiovascular, and neurodegenerative disorders. Although modern pharmacotherapy is effective, issues such as high cost, side effects, and limited accessibility—particularly in underdeveloped regions—have driven interest in herbal alternatives. Elixirs, as sweetened hydro-alcoholic solutions, offer an effective delivery system for herbal drugs. Objective To formulate and evaluate a herbal elixirs containing Crocus sativus (saffron) and Nigella sativa (black seed) for its in-vitro antioxidant and anti-inflammatory activities. Methods A hydro-alcoholic (40%) elixirs were prepared using different ratios of coarsely powdered C. sativus stigmas and N. sativa seeds, based on clinically used doses. Formulations were filtered after 48 hours and sweetened with 0.075 g sodium saccharin. Physicochemical parameters (viscosity and pH) were measured. Antioxidant activity was evaluated via hydrogen peroxide (H₂O₂) scavenging assay; anti-inflammatory activity was assessed through inhibition of egg albumin denaturation assay. Results Among all formulations, F2 elixir demonstrated significant antioxidant activity with 55 ± 0.23% inhibition at a 4 ml dose, comparable to ascorbic acid (56 ± 0.31% at 20 mg/ml). It also showed >50% inhibition of protein denaturation, with effects comparable to 60 µg/ml of diclofenac sodium. Conclusion The F2 herbal elixir exhibits potent antioxidant and anti-inflammatory activities in-vitro. These findings support that it could be cost-effective and accessible alternatives or adjuncts to conventional therapies for controlling oxidative stress and inflammation-related disorders.
Keywords	Anti-inflammatory, Antioxidant, Crocus sativus, Elixir, Nigella sativa.
Field	Medical / Pharmacy
Published In	Volume 16, Issue 2, April-June 2025
Published On	2025-06-22
DOI	https://doi.org/10.71097/IJSAT.v16.i2.6469
Short DOI	https://doi.org/g9q4cb

View / Download PDF File

doi

CrossRef DOI is assigned to each research paper published in our journal.

IJSAT DOI prefix is
10.71097/IJSAT

Downloads

Research Paper Format Copyright Permission Form and Undertaking Form Cover Page Vol 15 Isu 4 Cover Page Vol 15 Isu 3 Cover Page Vol 15 Isu 2

All research papers published on this website are licensed under Creative Commons Attribution-ShareAlike 4.0 International License, and all rights belong to their respective authors/researchers.

CC-BY-SA

About IJSAT Fees & Payment Current Issue Publication Archive	Submit Research Paper Track Submission Status Publication Guidelines Publication Ethics Peer Review & Plagiarism	Join as a Reviewer Editors & Reviewers Reviewer Referral Program Get Reviewer Membership Certi.	Website/Journal Policies Usage Policy Content Policies Privacy Policy

Contact Us	Message on WhatsApp	+91-9687-182-185	editor@ijsat.org

International Journal on Science and Technology

A Widely Indexed Open Access Peer Reviewed Multidisciplinary Bi-monthly Scholarly International Journal

In-vitro Antioxidant and Anti-Inflammatory Potential of herbal elixir containing Crocus Sativus and Nigella Sativa

Share this