Extraction & Isolation of Anthocyanin from Waste Hibiscus Flower using Column Chromatography and its Characterization

Author(s)	Ms. Ritika Saini
Country	India
Abstract	The large-scale disposal of flowers after cultural and religious ceremonies contributes significantly to environmental waste, with the UNFCCC reporting over 8 million tons discarded annually. Hibiscus rosa-sinensis, commonly used in such practices, is rich in phytochemicals including flavonoids, polyphenols, and anthocyanins. Anthocyanins are natural pigments responsible for the red to blue coloration in plants and are well recognized for their antioxidant, anti-inflammatory, anticancer, and cardioprotective activities. Their extraction from discarded hibiscus flowers not only provides a sustainable approach to waste management but also enables the utilization of these bioactive compounds in medicinal and industrial applications. The present study focuses on the extraction of anthocyanins from waste Hibiscus rosa-sinensis using acidified aqueous medium, followed by isolation through column chromatography and characterization via thin layer chromatography (TLC) and paper chromatography.
Keywords	Waste Hibiscus rosa-sinensis, Anthocyanin, Column Chromatography, Phytochemical activities, TLC.
Field	Chemistry > Pharmacy
Published In	Volume 17, Issue 1, January-March 2026
Published On	2026-02-16

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