International Journal on Science and Technology

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Volume 16 Issue 4
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Isolation and Characterization of Xanthomonas Species: A Plant Pathogen Causing Disease in Citrus Leaves

Author(s) Ms. Deepa Bansal
Country India
Abstract Plant pathogens such as Xanthomonas species are major contributors to agricultural losses, particularly in high-value crops like citrus. This study aimed to isolate and characterize Xanthomonas sp. from infected citrus leaves showing symptoms of necrotic spots, yellowing, and chlorosis. The pathogen was isolated using standard microbiological techniques, and its identity was confirmed through a combination of morphological, biochemical, and molecular approaches. Biochemical assays revealed key enzymatic activities and metabolic traits characteristic of Xanthomonas, while molecular tools, including PCR, provided precise species identification. Pathogenicity tests were conducted by inoculating healthy citrus plants, which resulted in symptom reproduction, confirming the organism’s virulence. These findings highlight the critical need for accurate and early identification of Xanthomonas sp. to manage and mitigate its impact on citrus crops. The study emphasizes the importance of integrating disease management strategies such as the development of resistant plant varieties, optimized agricultural practices, and targeted chemical control measures. By enhancing our understanding of the pathogen’s biology and behavior, this research provides a foundation for designing effective control strategies and contributes to improving agricultural productivity and sustainability. The outcomes have broad implications for plant pathology and underscore the urgency of addressing plant diseases in the context of global food security.
Keywords Xanthomonas species, plant pathogen, citrus leaves, bacterial disease, characterization, disease management
Field Biology > Agriculture / Botany
Published In Volume 16, Issue 4, October-December 2025
Published On 2025-10-10
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