Comparative Evaluation of Commercial Chemical and Traditional Natural Disinfectants by Phenol Coefficient Test and Antibacterial Assays

Author(s)	Dr. Nisy S, Ms. Banupriya P, Ms. Muheena C, Ms. Limsha L, Ms. Keerthy S Nair, Ms. Sneha P, Mr. Hariprasad S, Mr. Muhammed Thoufeeque S
Country	India
Abstract	Disinfectants play a crucial role in eliminating harmful microorganisms from various surfaces, ensuring hygiene and reducing infection risks. This study compares the effectiveness of chemical and natural disinfectants, specifically neem-based and citrus-based with neemleaf extract and citrus fruit juice with phenol coefficient test and antimicrobial assay. Efficacy of both commercial and natural disinfectants were tested with Klebsiella spp and Staphylococcus spp were isolated from the laboratory surfaces . The results indicates that neem and citrus-based commercial disinfectants show significantly higher antimicrobial efficacy than natural extracts. However, natural disinfectants present eco-friendly and non-toxic alternatives, despite lower effectiveness. The findings highlight the potential for developing improved herbal disinfectant formulations to reduce dependence on chemical-based solutions.
Keywords	Disinfectants, Neem, Lemon, Phenol Coefficient, Antibacterial
Published In	Volume 16, Issue 4, October-December 2025
Published On	2025-10-24
DOI	https://doi.org/10.71097/IJSAT.v16.i4.9022
Short DOI	https://doi.org/g98ncz

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International Journal on Science and Technology