Efficient Biodegradation of Poultry Feather Using Keratinase Nanoparticles

Author(s)	Prof. Venkata Lakshmi Vedula, Ms. Jhansi Rani K.P, Prof. Suvarnalatha Devi Pottireddy
Country	India
Abstract	Nanoparticles represent an emerging class of biocatalysts with improved stability, activity, and application potential in bioremediation, pharmaceuticals, cosmetics, and material sciences. In the present study, sustainable synthesis of silver nanoparticles (AgNPs) was synthesized using keratinase enzyme was produced from a novel keratin-degrading Bacillus species. The biosynthesized silver nanoparticle was characterized through UV–Visible spectroscopy, zeta potential measurement, particle size analysis, and UV–Vis spectra revealed maximum absorbance between 400–450 nm, confirming the formation of AgNPs. Average particle size of 490 nm to 582.2 nm, and zeta potential of −49.3 mV with a single peak. Keratinase Nanoparticles (KNP) showed high keratinase activity and biodegrading poultry feather. KNPs were stable even after 12 months. These findings demonstrate that keratinase-mediated synthesis of AgNPs is an eco-friendly strategy to stabilize the enzyme and extend the application potential of keratinase significantly in biotechnology, medicine, and environmental sustainability.
Keywords	Keratinase, Bacillus species, silver nanoparticles, biocatalysts
Published In	Volume 16, Issue 4, October-December 2025
Published On	2025-11-03
DOI	https://doi.org/10.71097/IJSAT.v16.i4.9210
Short DOI	https://doi.org/g99qk2

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International Journal on Science and Technology